Resources

Reagents

You can obtain plasmid and vector reagents via the nonprofit plasmid repository Addgene: Jin lab reagents at Addgene

If you cannot obtain reagents through Addgene or have additional questions, please feel free to reach out to us directly at jinoffice@scripps.edu.

Protocols

CRISPR-Cas9 editing efficiency analysis through next-generation sequencing

Whole-cell dissociation from perinatal mouse brains

Nuclei extraction from perinatal mouse brains

High-titer lentiviral vector production and purification

Software and Data

All the computational tools and pipelines are available on Github. We also share our data on the single-cell portal for easy access.

Jin lab Github | single cell portal

In vivo Perturb-seq (Jin et al. Science 2020)

GitHub | GSE157977 | single-cell portal

Massively parallel in vivo Perturb-seq (Zheng et al. Cell 2024)

GitHub | GSE249416 | single-cell portal: AAV screen (RNA, AAV barcode) and perturbation screen (RNA, gRNA) | the Neuroglancer portal of AAV-SCH9 transduced brain